Journal: The Journal of Pathology

Article Title: The YY1 / MMP2 axis promotes trophoblast invasion at the maternal–fetal interface

doi: 10.1002/path.4694

Figure Lengend Snippet: Knockdown of YY1 decreases the expression of MMPs in primary trophoblasts. (A) Primary trophoblasts were transfected with siCtrl or siYY1 oligonucleotides for 48 h, and the MMP and TIMP levels in the cell supernatant were quantified using Quantibody Human MMP Array 1. * p < 0.05 versus siCtrl. (B–G) MMP1, MMP2, MMP3, MMP 9, TIMP1 or TIMP2 expression levels in the supernatant of primary trophoblasts transfected with siCtrl or siYY1 were determined using an ELISA Kit (R&D Systems). (H, I) Serum‐free culture media of primary trophoblasts transfected as indicated with siCtrl, siYY1, vector, and YY1‐overexpession plasmid were collected for gelatin zymography. (J) The level of MMP2 expression in the supernatant of primary trophoblasts transfected with lenti‐ctrl or lenti‐YY1 lentivirus was determined using an ELISA kit (R&D Systems). (K) The level of MMP2 expression in the supernatant of the explants from RM patients incubated with lenti‐ctrl or lenti‐YY1 lentivirus was determined by ELISA.

Article Snippet: Human MMP antibody array analysis was performed using the Quantibody Human MMP Array 1 (RayBiotech Inc, Norcross, GA, USA) as detailed in the Supplementary methods.

Techniques: Expressing, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Zymography, Incubation